FastQCFastQC Report
Sun 14 Apr 2019
SRR6576182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221869
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4070.18344158039203315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.13431349129441247No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2590.11673555115856654No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2420.10907337212499267No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2380.10727050647003412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6800.042.1710661
GGACCGT450.001012209141.7775766
TACATGG15600.038.572612
GTACATG15650.038.4493751
ACATGGG16200.037.1356243
CATGGGC2200.036.318014
ATGGGTA1451.8189894E-1135.6550035
GTTACAC801.1544833E-535.2498323
ATGGGAG3950.034.5061655
CATGGGT3250.031.8152314
CATGGGA9400.031.499854
GATATAG600.00414932931.3402481
ATAGACT902.5846575E-531.3331834
AATGACG300.00415835131.33318386-87
ATAAGCC600.00415391131.3331833
ATCAACG9350.031.1656273
TCAACGC9450.030.3384784
TGGGTAC1551.5061232E-930.3224336
CAACGCA9550.030.0207985
AACGCAG9600.029.864446
Produced by FastQC (version 0.11.2)