FastQCFastQC Report
Sun 14 Apr 2019
SRR6576184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480255
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13230.27547865196614296No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7440.15491769997188995No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7260.15116969110160228No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7020.14617234594121872No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6820.1420078916408991No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6630.13805166005559547No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6590.13721876919553155No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT5540.11535538411885354No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5500.1145224932587896No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA5320.11077448438850195No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5290.110149816243454No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5260.10952514809840605No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5150.10723469823323026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.10515247108307045No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4840.10077979406773485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT8500.039.810444
ATGGGTA6650.039.5776335
GTATCAA13850.038.6967241
ACCGTAC550.002715883334.1806833
GGTACCT6050.034.180688
ACATGGG28650.033.4648553
GTACATG30500.033.14041
TACATGG31100.032.19872
TGGGTAC6850.030.8748876
GGGTACC6700.030.8646457
ATCAACG18000.029.7656763
AACGCAG17850.029.7525126
TCAACGC18000.029.5045744
CAACGCA18150.029.2607365
TAGGGGC650.00615164828.9221174
GTACCTG7450.027.7574679
CATGGGG11850.027.3661794
ACGCAGA19650.027.0270947
CGCAGAG19800.026.8223428
TAGTAGC909.511429E-426.1102436
Produced by FastQC (version 0.11.2)