FastQCFastQC Report
Sun 14 Apr 2019
SRR6576184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480255
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10750.22383941864217968No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7740.16116438142236938No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6590.13721876919553155No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6420.13367898304025985No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6400.13326253761022788No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5830.12139384285431698No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5710.1188951702741252No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5380.11202382067859783No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5190.1080675890932942No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4830.10057157135271887No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4820.10036334863770288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13100.044.2216641
CGCGATT250.00170105537.6037590-91
TCAACGC16650.034.4382864
AACGCAG16800.034.406986
GCATTAG550.00268788134.2531
ATCAACG16950.033.828763
ACATGGG29100.033.7558633
CAACGCA17050.033.6268465
GTACATG30100.033.3283921
TACATGG30800.032.50312
GTCTATA753.2330366E-431.398581
ATTAGCC600.004158681331.3331953
TATACGT600.004160801431.3299335
ACGCAGA19250.030.0279087
GATCTAA650.006088447828.9833051
TATCAAC20400.028.7988912
CGCAGAG20350.028.6357148
CATGGGT8250.028.4847244
CATTAGC856.8104453E-427.6469362
CATGGGG14050.027.0959664