FastQCFastQC Report
Sun 14 Apr 2019
SRR6576185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428606
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12690.2960761165266002No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7280.169852965194141No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6740.15725398151215803No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6520.1521210622343131No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6480.15118780418379585No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6480.15118780418379585No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5830.1360223608628904No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4980.11619062728939866No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4940.11525736923888139No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4820.11245759508732962No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4730.11035776447366578No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4660.1087245628852606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.1073246758094847No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4590.10709136129685538No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4590.10709136129685538No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4560.10639141775896745No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4400.10265838555689841No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4380.10219175653163978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.036.1482351
ATGGGTA4500.035.501465
ACATGGG26100.033.485153
GTACATG28100.032.7778321
TACATGG27850.032.565862
CATGGGT6250.031.5754154
GGTACCT4650.030.314348
CATGGGG11350.029.3928894
GTATAGG804.7802797E-429.3704411
AACGCAG15700.027.2346346
TCAACGC15600.026.8068164
ATCAACG15950.026.2185783
GTTCGCG459.369685E-426.18036846-47
CAACGCA16050.026.0552225
GGGTACC5300.025.7099917
ATGGGGA6150.025.2126585
TGGGTAC5800.025.1138636
GAATCCG950.001305317724.730129
CGCAGAG17400.024.5737788
ATGGGGC3450.024.5150765
Produced by FastQC (version 0.11.2)