FastQCFastQC Report
Sun 14 Apr 2019
SRR6576186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281762
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6430.22820678444928696No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4150.14728742697737807No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3860.1369950525620914No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3460.12279867405824774No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3230.11463575641853764No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3120.11073175232998063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7850.035.9286541
TAGTACA550.002714022834.180594
GTACATG16800.033.8559841
TACATGG17050.033.0838622
ACATGGG17050.032.802343
GTATGAG1950.031.3377721
CATATAG600.00415643131.332213
AACGCAG9050.031.1591056
TCAACGC9150.030.8185674
ATCAACG9450.029.8401993
CAACGCA9500.029.6831445
TCCAACG404.7788303E-429.37394722-23
GGTAAGG650.00614214128.9271742
GGTATCA4750.028.6988011
GTGCTAT1205.816275E-627.420551
ATGGGTA1904.4383341E-1027.209555
ACGCAGA10450.026.9846787
CGCAGAG10650.026.9192248
TACGCTA1755.516995E-926.8561789
CATGGGT3900.026.511874
Produced by FastQC (version 0.11.2)