FastQCFastQC Report
Sun 14 Apr 2019
SRR6576186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281762
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6250.22181841412255734No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3910.13876959987507187No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3320.11782994158190246No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3310.11747503211930636No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3260.1157004848063259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.10860229555440407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2980.10576301985363533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8550.042.431791
TAGACGT550.002713896834.1809165
AACGCAG9900.034.1809166
CAACGCA10150.032.875985
TCAACGC10300.032.3972054
ATCAACG10350.032.2406923
ACATGGG15150.029.7813913
ACGCAGA11750.028.799247
CATGGGT4250.028.752184
GTACATG16400.028.7291551
CGCAGAG12450.027.5575058
TACATGG16600.027.463132
GTGTAGC1554.935464E-827.357571
ACGGGAT350.00883340826.85643286-87
TGTAGCG700.00882604926.8564322
ATGGGAG2650.026.6030715
TATCAAC12950.026.4935072
TAGACTG909.501263E-426.110425
CCTCTCG909.501263E-426.110428
GCAGAGT14050.024.7537949
Produced by FastQC (version 0.11.2)