FastQCFastQC Report
Sun 14 Apr 2019
SRR6576187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249345
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6500.26068298943231266No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4120.16523291022478895No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3610.14477932182317674No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3240.12994044396318355No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3210.12873729170426518No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2820.11309631233832641No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2660.10667950029076179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7050.036.6640051
TACATGG13900.035.1629182
GTACATG13900.034.4867061
ACATGGG14300.033.1867373
AACGCAG8300.030.003856
TATAAGG1303.316054E-728.9209772
GAGCGGT650.006153259428.9151767
TATAAGC650.006153259428.9151764
ATCAACG8800.028.833033
TCAACGC8650.028.7898244
CAACGCA8800.028.2990885
GGTACCT2002.5465852E-1128.1922998
TCGTGAC350.00884308226.84980864-65
ATGGGGA3900.026.505585
CATGGGG6950.026.36694
CTAAGAC909.5118664E-426.1039813
TGGGTAC2357.2759576E-1225.99296
ACGCAGA9600.025.9408327
TCTAGGA1301.0839885E-525.3058552
ACTATAC1301.0856773E-525.300783
Produced by FastQC (version 0.11.2)