FastQCFastQC Report
Sun 14 Apr 2019
SRR6576190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260802
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3960.15183932638553385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.12653277198794488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7000.047.778821
TCAACGC8600.038.2537844
ATCAACG8800.036.850323
CAACGCA8850.036.6421245
AACGCAG9150.035.954386
TATCAAC9400.035.4981162
GTACATG14000.031.9647031
ACGCAGA10300.031.4837677
TACATGG14050.031.4431722
ACATGGG14100.031.331673
ATTAGAC600.00415621931.3316693
GCTATAG600.00415621931.3316697
CGCAGAG10400.031.181048
CATGGGG7000.028.1985024
GGTATCA3500.026.9176451
GGTACCT2450.026.8557198
GGGTACC2450.026.8557197
GCAGAGT12250.026.8557199
ATAGGGG700.00882601226.8557174
AACGGGC700.00882601226.85571794
Produced by FastQC (version 0.11.2)