FastQCFastQC Report
Sun 14 Apr 2019
SRR6576191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235562
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3250.13796792351907353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG200.002161613270.493631
GTATCAA5400.040.9037131
TACATGG12450.037.3621832
CATGGGT3150.037.29034
ACATGGG12450.036.607393
ATGGGTA2450.036.437955
GTACATG12900.036.066511
GGGTACC2050.034.3798377
TAATAGA550.002716673334.1714744
CAACGCA7000.032.218825
AACGCAG7000.032.218826
TGGGTAC2250.031.3238536
TCGCGTG300.00416467631.32385324-25
GGCCGTT753.2735072E-431.323857
ATCAACG7250.031.1078243
TCAACGC7250.031.1078244
ATGGGAG2850.029.675235
GGTACCT2400.029.3661128
CATGGGG6150.029.031864
TAACGCC650.006153466628.9143244
Produced by FastQC (version 0.11.2)