FastQCFastQC Report
Sun 14 Apr 2019
SRR6576191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235562
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3660.15537310771686436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1324492065783106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG200.002162058670.489972
GTATCAA6600.041.3410381
CTAATAG500.001700469537.594653
TACATGG13350.036.609022
GTACATG13400.036.160111
ACATGGG13750.035.544033
AACGCAG8250.033.6073386
TACTACA702.1815744E-433.566652
ATCAACG8300.032.83873
CAACGCA8650.031.5099665
TCAACGC8650.031.5099664
CATGGGG5900.031.0633754
TATCAAC8800.030.9728642
CATGGGT2900.030.7887234
TAACGCC804.7734557E-429.3708214
ACGCAGA9300.029.3076557
CGCAGAG9350.029.169518
TATATAC1005.3109798E-528.1959883
ACGTGCC350.00883695526.85332174-75
CTATACC909.5030345E-426.1073974
Produced by FastQC (version 0.11.2)