FastQCFastQC Report
Sun 14 Apr 2019
SRR6576193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361873
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10500.2901570440458393No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6600.1823844276859561No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5980.16525134508515418No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5870.16221160462372158No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5800.16027722433008265No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5510.15226336311357852No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4140.11440477736664521No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4130.11412843732469678No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4030.1113650369052126No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3950.10915431656962525No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3950.10915431656962525No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3950.10915431656962525No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3920.10832529644378No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3880.10721993627598632No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3870.10694359623403792No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3830.10583823606624422No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3810.10528555598234739No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3790.10473287589845057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG200.0021583970.5278852
ATTATAC450.001013129341.776983
GTATCAA9050.041.5633961
ATGGGTA4050.039.4560365
GGGTACC3750.038.8525927
CATGGGT5550.037.2605484
GGTACCT3950.036.8853728
GTACATG21650.035.8340341
TGGGTAC4100.035.5359046
TACATGG21450.035.5105482
ACATGGG21550.035.3311163
TATATAC550.00271481434.1811684
ATGGGGG4000.034.074355
CATGGGG10900.032.338834
TATAGAC753.271508E-431.3327354
ACGTAGG1052.0605075E-631.33273394
CAACGCA11600.031.197685
TCAACGC11600.031.197684
AACGCAG11450.031.195916
ATCAACG11650.031.0637843
Produced by FastQC (version 0.11.2)