FastQCFastQC Report
Sun 14 Apr 2019
SRR6576193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361873
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10070.2782744222420573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6230.17215984613386465No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5850.16165892453982475No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5490.1517106830296817No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5240.14480218198097122No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5080.14038074130979653No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4290.11854987799587147No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4210.11633915766028413No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3960.10943065661157367No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3820.10556189602429582No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3790.10473287589845057No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3720.10279849560481163No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3690.10196947547896637No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3620.10003509518532745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.041.685611
GTACATG22400.037.2089541
TACATGG22350.037.0148852
ACATGGG23250.035.1777083
CCTAATA550.00271322934.1852382
CATGGGG13300.033.2213674
AACGCAG12000.032.511586
CAACGCA12050.032.3766825
TCAACGC12200.031.5933234
ATCAACG12250.031.4643693
GCTATAA600.004118797431.3928641
ACGTAGG902.5897298E-531.33213494
GTCTTAG1401.8490027E-830.2716921
ATGGGTA3750.030.0830065
TACCGTC1354.62147E-727.8507867
GGGTACC3550.027.8017547
GGTACCT3750.027.5722778
TGGGTAC3800.027.2132456
CATGGGT5650.026.622134
TATCAAC14750.026.45012
Produced by FastQC (version 0.11.2)