FastQCFastQC Report
Sun 14 Apr 2019
SRR6576196_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576196_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248048
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4150.16730632780752114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.13586080113526414No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2900.11691285557634006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2670.10764045668580276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.038.8887941
CTAGGAC757.424953E-637.596373
TACATGG15400.036.6198432
GTACATG15600.036.48841
ACATGGG15400.035.7043463
CTATACT702.1813193E-433.5681884
AACGCAG9200.033.2033166
ATGGGGG2600.032.535325
GTCGCGT902.569713E-531.3619394
GTATAAG753.2507497E-431.3619291
CATGGGG5900.031.0647964
TCAACGC9700.030.5228274
ATCAACG9550.030.0180443
CAACGCA10100.029.7793035
ATGGGTG2002.5465852E-1128.1972775
TATATCT1005.3104406E-528.1972774
ACGCAGA10600.027.9312637
CATGGGA8200.027.5095394
TGGGAGT2253.6379788E-1227.1529336
CGCAGAG10950.027.0548538
Produced by FastQC (version 0.11.2)