FastQCFastQC Report
Sun 14 Apr 2019
SRR6576197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116860
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.2130754749272634No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1440.12322437104227281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA4900.051.7959181
GGGATGT555.253899E-542.7272727
AACGCAG6300.040.2857176
ATCAACG6450.040.0775223
CAACGCA6500.039.7692345
GTACATG10700.039.093461
ACATGGG10600.038.5754743
ATGGGAG2450.038.3673485
TCAACGC6750.038.2962954
TACATGG10850.038.1198162
ACTATCT757.3864303E-637.6000028
TAGGGGT500.00169577837.6000024
GAGATCA500.00169577837.6000029
CATGGGG2750.037.64
TATCAAC7100.036.408452
CATGGGA6900.034.7391324
CGCAGAG7350.034.5306138
ATGGGGA1107.595918E-834.1818165
ACGCAGA7450.034.0671167
ATGGGTG851.7413902E-533.1764685
Produced by FastQC (version 0.11.2)