FastQCFastQC Report
Sun 14 Apr 2019
SRR6576197_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576197_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116860
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.250727366079069No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1460.12493582064008214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.11552284785213075No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1170.10011980147184665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG255.5388777E-575.1989755
CGATGCT200.002158223270.499044
GACTCCG200.002158223270.4990494
GTATCAA4600.062.4053421
GTACATG11200.043.278181
ATGGGAT2500.043.239415
ATCAACG6600.042.726693
TACATGG11450.042.6893732
ACATGGG11300.042.42423
TCAACGC6750.041.777214
CAACGCA6800.041.4700245
TATCAAC6950.041.2512322
AACGCAG6750.041.080926
ATGGGAG3100.039.4188165
CATGGGA7500.038.2261434
GGGAGAG500.001695891837.5994877
CGCAGAG7500.036.9728288
ACGCAGA7600.036.4863437
CATGGGC1651.8189894E-1234.181354
ATGGGGG1256.166374E-933.839545
Produced by FastQC (version 0.11.2)