FastQCFastQC Report
Sun 14 Apr 2019
SRR6576198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.24432268827889256No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1370.12489629960525477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG405.6648476E-446.9819875
GTCCTAC652.7604383E-643.3877751
GACCGCG450.001011849741.761777
GTACATG11450.041.4615331
TACATGG11550.040.6956062
ACATGGG11500.040.0368273
GTATCAA5350.039.535591
CATGGGA7100.037.7179374
GTACAAG500.001694712637.6027371
ATGGGAT2550.035.006195
GTATTAA550.002705381734.1843071
CATGGGG3450.032.683124
AACGCAG6500.031.8031926
CATGGGT1351.3089448E-831.3213254
TATACTG753.263732E-431.3213255
AACCAGT600.0041525231.3213236
AATGCGT300.0041615131.32132358-59
TCGTATT300.0041615131.32132372-73
TATCAAC7150.030.2399642
ATCAACG6950.029.7439923
Produced by FastQC (version 0.11.2)