FastQCFastQC Report
Sun 14 Apr 2019
SRR6576198_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576198_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109691
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.28717032390989233No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1920.1750371498117439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.1349244696465526No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1470.13401281782461644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.11942638867363775No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1160.10575161134459528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT200.002160742270.475383
CTACTTA250.0052306856.38034
CTTATGA352.9176468E-453.7445379
GTATCAA5950.042.7184831
GGACTGA450.001009411141.7822386
GTACATG12050.036.7180671
TACATGG12100.036.4996452
ACATGGG12300.035.9061553
ACAGGGG801.1518863E-535.237693
ATGGGAG2750.034.169885
TCTTACA550.002710992734.169882
TAGGGCT550.002710992734.169884
CATGGGG3600.033.9325874
CATGGGC1703.6379788E-1233.1648834
ATCAACG7400.033.015493
ATGGGGG1151.12488124E-732.684235
TCAACGC7500.032.5752834
AACGCAG7650.032.565576
CATGGGA7200.031.9749374
CAACGCA7800.031.9247425
Produced by FastQC (version 0.11.2)