FastQCFastQC Report
Sun 14 Apr 2019
SRR6576199_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576199_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330127
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8740.2647465975215599No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7070.2141600050889506No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6470.19598518146046826No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5240.1587267930220794No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5040.15266851847925192No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4840.14661024393642447No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4780.14479276157357623No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4420.1338878673964868No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4220.12782959285365936No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4160.12601211049081112No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4080.12358880067368014No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3860.11692469867656992No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3850.11662178494942854No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3780.11450138885943896No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3630.10995768295231835No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3590.10874602804375286No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3530.10692854568090462No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3470.10511106331805638No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3450.10450523586377364No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC3330.10087027113807716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC250.0052306456.4057273
GGGTACC3850.041.504427
GGTACCT3850.040.2837038
GTATCAA9500.035.225941
GTACCTG4300.034.9749959
ATGGGTA4850.034.890145
CATGGGT6300.033.5748374
GTACATG18700.031.7582631
TGGGTAC5200.031.637836
TCTAATA600.00415465931.3365152
AATACCT753.268578E-431.3365155
ACATGGG18900.031.0878123
TACATGG19800.029.6747282
ATACCTT804.7707508E-429.3779816
AACGCAG11500.028.2028646
CATGGGG7700.028.0807744
ATCAACG11250.027.5761343
TCAACGC11450.027.0944544
CAACGCA11650.027.0327875
GGTATCA5100.026.8012871
Produced by FastQC (version 0.11.2)