FastQCFastQC Report
Sun 14 Apr 2019
SRR6576201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460674
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10140.22011227028223865No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5430.11787077195587335No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5250.11396345354849632No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4800.10419515753005379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC906.179944E-736.555263
GTATCAA13900.035.1651841
GTACATG27500.031.7888361
TACATGG27450.031.6755162
GTACTAG600.00415858231.333081
TACCGTG600.00415858231.333087
ACATGGG26900.030.9254023
CATGGGT6600.030.6209644
TCAACGC16700.028.987794
AACGCAG17000.028.7527086
CAACGCA17050.028.392735
ATCAACG16900.028.3666323
ATGGGTA4500.028.1997725
CATGGGG12100.027.9667174
TGGGTAC4250.027.6468336
GGGTACC4450.027.4604537
GGTACCT4250.026.540968
ACGCAGA19400.024.9534057
CGCAGAG19750.024.7491668
TATCAAC20500.024.3022442
Produced by FastQC (version 0.11.2)