FastQCFastQC Report
Sun 14 Apr 2019
SRR6576202_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576202_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417138
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8730.20928325877767068No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5650.13544678259952342No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5210.12489871457407381No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5010.12010413819886945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.10883688371713918No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4360.10452176497945523No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4290.10284366324813371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.042.8502581
GTACATG24350.034.7867661
TACATGG24600.034.7699172
ACATGGG24100.034.7112583
AACGCAG14800.034.294896
CATGGGG9750.034.223244
TCAACGC15150.033.812814
ATCAACG15200.033.7015843
CAACGCA15600.032.837445
TATCAAC17800.029.3069322
CATGGGT6100.029.2766344
CGCAGAG17350.029.0009428
ACGCAGA17350.028.9800837
CTACGTC650.006151843428.9210453
TGGGTAC3750.027.5713986
GGGTACC4000.027.0198657
ACCGCGA350.00883251526.85847940-41
ATGGGAG5100.026.7236145
ATGGGTA4450.026.4026435
GGTACCT4100.026.3798168
Produced by FastQC (version 0.11.2)