FastQCFastQC Report
Sun 14 Apr 2019
SRR6576203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334503
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9350.2795191672421473No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5290.15814506895304375No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5290.15814506895304375No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4670.13961010813056984No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4280.1279510198712717No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4240.12675521594724112No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4220.12615731398522584No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3900.11659088259298124No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3780.1130034708208895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.10702445120073661No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3560.10642654923872133No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3420.1022412355046143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA255.5546672E-575.1971363
GCAAACG250.005211950756.4569241
ATACGCG250.005233593856.397856
CCGCTAA555.276224E-542.7256434
GTACTAG450.001007907441.819941
CGCTAAA608.8126515E-539.1651735
GGTACCT4000.037.5985688
GGGTACC4000.037.5985687
GTATCAA12550.035.2387121
TGGGTAC4550.034.0866136
CATGGGT5750.031.8770454
ATGGGTA4750.030.6725145
GTACCTG5050.029.781049
TACATGG21300.029.5802122
AACGCAG14750.029.3141376
ATCAACG14850.029.1167343
GTACATG22400.028.7745481
CAACGCA15050.028.72985
TATACAG1154.185955E-628.6076035
TCAACGC15150.028.5401654
Produced by FastQC (version 0.11.2)