FastQCFastQC Report
Sun 14 Apr 2019
SRR6576203_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576203_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334503
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9920.29655937315958303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5690.17010310819334953No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5590.1671135983832731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.163825137592189No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5080.15186709835188325No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4930.14738283363676857No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4570.1366205983204934No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4380.13094052968134817No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4250.12705416692824878No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3770.11270451983988186No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3750.11210661787786656No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3620.10822025512476718No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3530.10552969629569839No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3500.10463284335267546No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3350.1001485786375608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGT450.001013264841.774924
GTATAAC550.002661857334.317981
CTTACAC702.1825808E-433.5691343
ACCTAAG1703.6379788E-1233.308631
TTAGGTA1001.407956E-632.8977474
ATACCAT753.2719283E-431.3311926
GTATCAA15100.030.624821
CTAAGAC2150.030.602563
GGGTACC4000.030.5479097
TCTTAGG1701.2187229E-1030.418782
GGTACCT3900.030.1261468
GTATAAG650.00602972229.0382941
CCTAAGA1951.8189894E-1128.9297492
TTCCGAC1354.6209243E-727.8499497
TAAGACA3450.027.2445134
TAGGTAG700.00883017326.8553097
CCTATAT700.00883017326.8553093
TGGGTAC4550.026.8553076
CTTAGGT1057.409573E-526.8553053
GTACATG24400.026.1076871
Produced by FastQC (version 0.11.2)