FastQCFastQC Report
Sun 14 Apr 2019
SRR6576204_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576204_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229584
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5600.2439194368945571No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3780.16464561990382603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.14896508467489025No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3290.14330266917555232No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3240.14112481706042235No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3060.13328454944595444No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2810.12239528887030456No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2580.11237716914070668No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2510.10932817617952471No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2490.10845703533347271No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2340.1019234789880828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6800.047.9178161
ATGGGTA2850.046.1826065
GGGTACC3050.041.603957
GGTACCT3000.040.7307858
CATGGGT3950.040.4619754
ATGGGCG704.6141013E-640.291975
GTACATG13250.039.91711
TGGGTAC3300.038.4605146
TACATGG14000.037.9416052
ACATGGG13900.036.861843
GTACCTG3450.035.4180769
CAACGCA9300.034.876385
ATCAACG9200.034.7445223
AACGCAG9250.034.5567176
TCAACGC9300.034.3709264
ATTTAGG550.002650150334.3442541
AAGCTAA550.002713334534.179689
CATGGGC3400.031.7990554
ACGCAGA10550.029.8464747
AACGCCG953.735388E-529.688825
Produced by FastQC (version 0.11.2)