FastQCFastQC Report
Sun 14 Apr 2019
SRR6576205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211308
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5410.25602438147159595No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3630.1717871542960986No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3390.16042932591288545No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3270.15475041172127887No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2920.13818691199575975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.13298124065345374No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2780.1315615121055521No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2580.12209665511954114No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2380.1126317981335302No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2380.1126317981335302No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2370.11215855528422965No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2350.11121206958562857No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2350.11121206958562857No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2160.10222045544891817No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2150.10174721259961761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAACG250.005235254856.3860863
GTATCAA6900.049.7123681
AACGCAG8850.038.7587976
GTACATG16000.037.003371
CAACGCA9100.036.661285
ATCAACG9250.036.5747573
TCAACGC9350.036.1835864
TACATGG16600.035.6658972
ACATGGG16600.035.382833
ATATGAC550.002715298734.1733863
CTACACT550.002715298734.1733864
ACGCAGA10150.033.7946177
CATGGGA7050.032.6586074
CGCAGAG10600.032.359948
CATGGGG7350.031.3256034
TTATGGA600.00415838931.3256034
ATAGTCT600.00415838931.3256036
ATGGGAG3500.030.8780945
ATGGGTA2500.030.072585
TATCAAC11450.029.9576722
Produced by FastQC (version 0.11.2)