FastQCFastQC Report
Sun 14 Apr 2019
SRR6576206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232247
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3560.15328508010867742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.13218685279034822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT250.005231933756.39689694
GTATCAA7200.050.9358021
AACGCAG8850.040.8904086
CAACGCA9150.039.5497365
TCAACGC9150.039.5497364
ATCAACG9250.039.1221733
TGCCCGC500.001696097337.614131
ACGCAGA9850.036.7390947
TATCAAC10250.036.2380072
CGCAGAG10100.035.364398
GCAGAGT11400.031.331619
GTACATG12750.030.9763411
TACATGG12800.030.8553412
ATGGGAG2600.030.729085
ACATGGG12750.030.5943953
CATGGGT3650.029.6148134
ATGGGTA1751.70985E-1029.5412315
GGTACCT1652.9394869E-928.4832828
CATGGGG5050.027.9192584
GGGTACC1704.0436134E-927.645547
Produced by FastQC (version 0.11.2)