FastQCFastQC Report
Sun 14 Apr 2019
SRR6576206_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576206_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232247
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.14036779807704727No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3130.13477030919667424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6950.044.0865171
ACATGGG14150.036.5501673
CATGGGT3300.035.618794
TACATGG14700.035.5024832
AACGCAG8650.034.7876
GTACATG14850.034.6000981
TCAACGC8700.033.506234
CAACGCA8850.033.469595
ATCAACG8800.033.1254773
ATGGGTA1850.033.0388345
GTATAAG1151.1017801E-732.7921141
TATCAAC9900.029.9197852
CTAGTAC650.006133471628.9334183
CGCAGAG10350.028.6127598
ATGGGAG4300.028.4287645
CATGGGG5300.027.5003954
ACGCAGA10850.027.2941977
GTACTTT7350.026.9363771
CATGGGA7700.026.8667454
GGTATCA3750.025.140621
Produced by FastQC (version 0.11.2)