FastQCFastQC Report
Sun 14 Apr 2019
SRR6576207_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576207_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207432
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.17981796444135908No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3200.1542674225770373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7350.045.4690131
AACGCAG8750.038.1295136
ATCAACG9000.037.0703623
TCAACGC9100.036.6629944
CAACGCA9250.036.068465
TATCAAC9750.034.717492
TACATGG13200.034.1914632
ACATGGG13700.033.2707183
GTACATG13850.032.9660151
ACGCAGA10300.031.9353567
CATGGGG4450.031.6790544
CGCAGAG10400.031.6511768
CGATTAC300.00414730331.3497472-73
GCAGAGT11200.029.8102429
GTCTAAC650.00609816328.9661711
CATGGGA6900.028.6029744
GGTATCA3250.027.5178641
ATAGACT700.00882824726.851774
CATGGGT2850.026.3806864
GTACTTG909.409131E-426.1500191
Produced by FastQC (version 0.11.2)