FastQCFastQC Report
Sun 14 Apr 2019
SRR6576208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298354
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4190.14043719876388452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.11764548154206077No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3050.10222755518612119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC555.275601E-542.7246068
GTATCAA9550.041.3515931
TAATACG250.001701893537.59765230-31
ATCAACG11450.034.47823
AACGCAG11550.034.1796886
ACATGGG18350.034.0632673
CAACGCA11600.034.032365
TACATGG18250.034.0037962
TCAACGC11750.033.9978754
GTACATG18150.033.932121
TAGTACT1151.13313945E-732.693614
GTCCTAC902.5827187E-531.3418851
TATCAAC13000.030.7391572
ACGCAGA13600.029.3731677
GTATAGG650.00613877528.930971
GGTATCA5450.028.4664821
CGCAGAG14100.027.6649388
CATGGGG6250.027.070314
CATGGGA9950.026.9229434
ATAAGGG700.008828416526.8554673
Produced by FastQC (version 0.11.2)