FastQCFastQC Report
Sun 14 Apr 2019
SRR6576208_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576208_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298354
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.14077237107597015No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4070.13641513101885677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9000.043.5004271
AACGCAG12250.031.8476
TACATGG17400.031.6055342
ATCAACG11900.031.5987243
CAACGCA12150.031.33545
GTACATG18000.030.6599391
TCAACGC12300.030.5711214
CATGGGA10350.029.5188544
ACATGGG18150.029.2636413
GTCTATA650.00603999829.027161
CGCAGAG14100.027.6638578
ACGCAGA14150.027.233987
TATCAAC14150.026.9063662
AATAGTC909.506747E-426.108467
TACTAGG950.001301822624.7384742
GCAGAGT16200.024.07789
CATGGGG6900.023.8421554
ATGGGAT3550.023.832565
AGTACTT7400.023.81514712-13
GTGTTAG1000.00171931123.5845681
Produced by FastQC (version 0.11.2)