FastQCFastQC Report
Sun 14 Apr 2019
SRR6576209_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576209_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275282
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4300.15620345681882578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.1394933195777421No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2880.10461998968330657No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2850.10353019812410545No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2830.10280367041797138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.047.6425861
CCGTCGT502.996321E-547.018819
ATACCGT601.6022568E-646.9931876
TACCGTC555.2771E-542.721087
ATCAACG10550.036.080083
TCAACGC10650.035.74134
CAACGCA10950.034.762085
ATTAGCC550.002715365334.1768653
AACGCAG11150.034.138556
ACCGTCG702.1755825E-433.5848668
GGTACCT1606.002665E-1132.325438
TATCAAC11800.032.2580342
TACATGG17600.030.9727842
GTACATG18050.030.766021
ATGGGTA2150.030.6002165
ACATGGG18250.029.6121483
CGTCGTA404.7665002E-429.38675910-11
GGCGTTA404.7817398E-429.37074352-53
CTAATAG650.00615050528.9188822
CGCAGAG13650.027.901278
Produced by FastQC (version 0.11.2)