FastQCFastQC Report
Sun 14 Apr 2019
SRR6576210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493528
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11440.23180042469728163No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7860.15926148060495046No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7600.1539932891345577No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7350.1489277204130262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.12238414031220113No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5890.11934479907928223No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5790.11731857159066963No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5530.11205038012027685No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5400.10941628438508048No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5230.10597169765443905No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5130.10394547016582646No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5040.10212186542607513No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5030.10191924267721386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13700.047.46941
TCAACGC16400.038.4034774
AACGCAG16750.038.1622246
CAACGCA16550.038.0554055
ATCAACG16700.037.7135933
ATGGGTA4850.035.8566365
GGTACCT4500.034.4641048
ACGCAGA18550.034.455667
CATGGGT7100.034.4234664
GTACATG27450.033.9921041
CGCAGAG18850.033.907298
ACATGGG27200.033.868563
TACATGG27650.033.6573262
GGGTACC5050.031.641217
TATCAAC21250.030.5231742
AAGACCG650.006150002628.9238575
GCAGAGT22700.028.363539
TGGGTAC5750.026.974646
GTATTAC1057.279865E-526.9288121
CATGGGG9900.026.1118164
Produced by FastQC (version 0.11.2)