FastQCFastQC Report
Sun 14 Apr 2019
SRR6576211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450598
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11280.2503340005947652No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7420.16467006067492532No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6660.14780358545754751No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6600.14647202162459666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.135375656350006No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5440.12072845418754632No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5400.11984074496557907No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5130.11384870771730012No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4980.11051979813492294No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4930.10941016160746386No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4910.10896630699648022No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4830.10719088855254573No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4800.10652510663607029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10750.046.838591
CCCTATA608.821304E-539.162322
GTACATG25000.038.9635731
TACATGG25750.037.595832
ACATGGG25000.037.4078453
ATCAACG13050.037.091673
AACGCAG13550.036.0697946
TCAACGC13600.035.9371874
CAACGCA13700.035.6748735
CATGGGA10900.034.0604364
ATGGGTA4850.031.9758345
GTCTTAC600.004133525331.3716411
ATGGGAT3750.031.3298575
ACGCAGA16000.030.5432197
CGCAGAG16500.029.6374028
CCTATAC804.779427E-429.371743
CATGGGT7800.028.9198674
TATCAAC17700.028.1437682
CATGGGG9450.027.8487594
GTGTACG700.00877795626.8899771
Produced by FastQC (version 0.11.2)