FastQCFastQC Report
Sun 14 Apr 2019
SRR6576213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264225
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8180.3095846343078816No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.21004825432869711No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4130.15630617844639985No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3960.14987226795344877No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3610.13662598164443182No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3280.12413662598164442No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3200.12110890339672627No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3130.11845964613492288No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3100.11732425016557858No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3050.11543192355000471No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3000.11353959693443089No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2980.11278266628820133No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2920.11051187434951272No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2830.10710568644147979No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2810.10634875579525027No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2760.1044564291796764No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2730.1033210332103321No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC2720.10294256788721734No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG2680.10142870659475825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA200.002163496870.480553
GTATTAC250.005229462456.4057921
AATAGTA352.9373623E-453.699475
GTATCAA7700.049.4466361
TATTACC503.0012628E-547.004832
AACGCAG8350.043.8920826
CAACGCA8700.042.6663865
TCAACGC8900.041.7075964
ATCAACG8950.041.474593
CCCTACA608.800303E-539.170692
GATAATA500.001698797737.6038632
GATATAG500.001698797737.6038631
GTACATG14950.037.10081
ACGCAGA9900.037.020097
TACATGG14750.036.647832
CGCAGAG10150.035.6453368
ACATGGG15650.035.4279253
GGTACCT2950.035.041188
ATATAGA550.002711872634.185332
TACTACG550.002716912434.172395
Produced by FastQC (version 0.11.2)