FastQCFastQC Report
Sun 14 Apr 2019
SRR6576215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404877
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8300.20500053102547192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.12102440988250754No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4580.11312077495140499No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4390.10842799171106286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA250.005234441356.3977055
ACCGTAC250.005234441356.3977053
GTATCAA10950.046.795041
GTACATG23800.037.1337131
TCAACGC13700.036.7065354
ATCAACG13800.036.4405443
TACATGG24100.035.8912242
ACATGGG23950.035.7146223
AACGCAG14250.035.6196026
CAACGCA14350.035.043875
CGTATGA300.004153549731.34367276-77
TACCGTG600.004158655631.332067
CATGGGT5550.031.3320584
GGGTACC3150.031.3320587
TACGCTC753.2726055E-431.33205694
ATGGGAG5450.031.044615
TATCAAC16550.030.961072
TGGGTAC3200.030.8424936
CGCAGAG16700.030.3939728
ACGCAGA17000.029.8576077
Produced by FastQC (version 0.11.2)