FastQCFastQC Report
Sun 14 Apr 2019
SRR6576215_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576215_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404877
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7620.18820530679687905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.1281870790388192No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4550.11237980917661414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.047.8007351
GTATACG500.00167148337.7329671
ATCAACG14150.036.880793
CAACGCA14550.036.5131385
AACGCAG14800.035.8963626
TCAACGC14600.035.7440574
GTACATG22200.031.6566031
GGCGTCG600.004159005431.331528
CGCGATT300.004161440331.3315278-79
TACATGG22150.031.201632
TATCAAC17500.030.8953742
CGCAGAG17100.030.7818458
ACGCAGA17150.030.6921047
ACATGGG22750.029.9654143
TAGTGCG650.006151262728.9214047
CATGGGT4600.028.6176454
TAATACC1005.3038177E-528.208824
CCGACCA1005.3172334E-528.1983689
GCAGAGT19700.026.9578349
TAGTTCC1755.513357E-926.8655454
Produced by FastQC (version 0.11.2)