FastQCFastQC Report
Sun 14 Apr 2019
SRR6576216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358082
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7300.20386391943744728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.12399394552085835No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4050.1131025854413235No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3920.10947213208147855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9450.043.7671281
ACATGGG19200.040.8687323
TACATGG19500.040.4922452
GTACATG19950.040.2856561
AACGCAG10050.039.740086
ATCAACG10650.037.5012053
CAACGCA10850.036.8099375
CATGGGG8000.035.827444
TCAACGC11200.035.6596264
GTAATAG1256.2409526E-933.839951
ACGCAGA12350.032.3390967
CATGGGA9700.031.9704024
CGCAGAG12550.031.8237328
ACTATAT600.004162908531.3245353
TATCAAC13400.029.8133892
TGCCCGC804.7744258E-429.3749581
CATGGGT4600.027.5792124
ATGGGAG4400.026.6970485
CGACGGT909.5204305E-426.103787
GCAGAGT15300.026.1037799
Produced by FastQC (version 0.11.2)