FastQCFastQC Report
Sun 14 Apr 2019
SRR6576216_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576216_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358082
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6880.19213476242871744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.15303757239961796No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3840.10723800693695858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.048.7366451
AACGCAG14300.036.8076326
ATCAACG14400.036.5520253
CAACGCA14700.036.125765
TCAACGC14600.036.051314
GTACATG20500.035.108991
TACATGG20800.034.5687752
ACATGGG20600.034.2199973
ACGCAGA15800.033.011197
TATCAAC16450.032.853972
CGCAGAG16350.031.9274758
CATGGGA8900.029.5701774
CATGGGG8150.029.4082034
GCAGAGT18700.027.6637089
GGTATCA5500.026.5142771
TCTAGAC1659.288851E-825.6338883
ATGGGAG4750.024.7344535
GTAGGAC1151.3755244E-424.519373
CATGGGT5450.023.2821524
CATGGGC4050.023.2076364
Produced by FastQC (version 0.11.2)