FastQCFastQC Report
Sun 14 Apr 2019
SRR6576217_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576217_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764236
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16280.21302320225689447No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10770.14092505456429688No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9860.12901773797622723No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA9490.12417630156129783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.12404545192846189No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8140.10651160112844724No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG7850.10271696177620525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18350.044.654351
GTACATG41800.034.474321
ACATGGG40700.034.2994963
TCAACGC23750.034.0378464
ATCAACG24050.033.8108983
TACATGG42550.033.6919362
AACGCAG24500.033.571386
CAACGCA24650.033.3670925
CATGGGT11150.031.1927814
TAGGTAT953.7506838E-529.6841685
ACGCAGA28700.028.492877
ATGGGTA8750.028.4685335
CGCAGAG30000.027.2581798
TATCAAC30300.026.9918272
CATGGGG20550.026.7590874
GGTACCT8050.025.6877258
TCTATAC1101.0174558E-425.6380063
GGGTACC8600.025.1378547
ATACCGT1501.1616921E-625.066636
CATGGGA14850.025.0033324
Produced by FastQC (version 0.11.2)