FastQCFastQC Report
Sun 14 Apr 2019
SRR6576218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689040
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17410.2526703819807268No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10910.15833623592244284No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10140.14716126785092304No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10120.14687100893997446No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8480.12306977824219205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.1155230465575293No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7650.11102403343782655No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7400.10739579705096947No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7090.1028967839312667No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT6990.10144548937652387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16150.045.6905481
GTACATG39050.036.2280241
TACATGG39750.036.0630042
ACATGGG38550.035.7121773
AACGCAG21800.032.9767656
ATCAACG21950.032.9654773
TCAACGC21950.032.9654774
CAACGCA22600.032.0173535
CATGGGT9850.031.0063194
ATGGGTA7150.030.2290785
GGTACCT6400.030.1007278
CATGGGG19350.029.8673884
ACGCAGA25300.028.414767
TATTCCG856.8247475E-427.6391185
TATCAAC27150.027.3518332
GGGTACC7300.027.033337
CGCAGAG26800.026.9997068
TAGGCGC700.00884599326.8494284
ATGGGGA10000.025.8425755
CATGGGA14950.025.771864
Produced by FastQC (version 0.11.2)