FastQCFastQC Report
Sun 14 Apr 2019
SRR6576218_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576218_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689040
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16320.23685127133402997No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10680.1549982584465343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.1396145361662603No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8660.12568210844072913No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8250.11973180076628352No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7940.11523278764658075No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7880.11436201091373506No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG7770.11276558690351793No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7070.10260652502031811No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6950.10086497155462673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18350.045.650571
ATCAACG23650.035.17013
TCAACGC23850.034.875174
AACGCAG23750.034.6262866
GGTACCT6800.034.5761228
ATGGGTA6650.033.919635
ACATGGG41700.033.807783
GTACATG41900.033.807631
CAACGCA24400.033.703865
TACATGG42650.033.1673242
CATGGGT10800.030.8934234
CATGGGG19900.030.4626774
ACGCAGA27600.029.7961677
GTAATAC1751.70985E-1029.538343
TGGGTAC7750.029.1052276
GGGTACC7750.029.1052277
CGCAGAG28550.028.9882348
TAATACC1802.382876E-1028.7178334
TATCAAC29350.028.6620922
GTACCTG8700.026.4845149
Produced by FastQC (version 0.11.2)