FastQCFastQC Report
Sun 14 Apr 2019
SRR6576219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182068
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3280.1801524705055254No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1840.1010611419909045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2900.056.724141
GTACTAG405.664065E-447.01
ATCAACG4200.040.2857133
TCAACGC4200.040.2857134
CAACGCA4250.038.7058835
AACGCAG4550.036.1538476
ACTAGGC550.002711034134.1818163
GTCTTAT550.002711034134.1818161
TATCAAC5150.031.9417462
ACCGGCG600.004151873331.3333348
GTATAAA753.2648075E-431.3333341
ACGCAGA5450.031.045877
GTACATG8450.030.5917171
CGCAGAG5900.029.4745778
ATACTGC804.7652744E-429.3756
TAACCCT804.7652744E-429.3754
AGTAGTA804.7652744E-429.3752
CTGGTCG650.006140757428.9230779
TTATTGC650.006140757428.9230774
GTAGTAT650.006140757428.9230773
Produced by FastQC (version 0.11.2)