FastQCFastQC Report
Sun 14 Apr 2019
SRR6576219_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576219_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182068
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3490.19168662258057428No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2450.13456510754223697No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2150.11808774743502427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2750.065.122421
GTATAGA652.720517E-643.502831
CATGGGG4450.041.1898044
ATCAACG4450.040.1336563
CAACGCA4500.039.687735
TCAACGC4550.039.2516024
GTACATG7250.039.0025371
AACGCAG4700.038.998866
TACATGG7500.037.59892
TAGGACT959.39488E-734.6305664
ACATGGG8150.034.6002163
ATTGTAC550.002711422334.180823
ACGCAGA5550.033.0260627
CGCAGAG5750.031.8773298
GTACAGA600.004096918731.418711
GTATAAT753.213092E-431.418711
TATCAAC5550.031.3324182
ATGGGGA2400.031.3324155
GGACCAT600.00415246831.3324156
GGTATCA1154.083453E-628.6866471
Produced by FastQC (version 0.11.2)