FastQCFastQC Report
Sun 14 Apr 2019
SRR6576220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152205
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2870.1885614795834565No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1710.11234847738247758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAT250.005233058256.38442694
TACCGTA250.005233058256.3844267
GTATCAA3350.054.7192881
CCTATTG450.001012556741.7662433
GAATAAC608.785313E-539.168721
ATCAACG4650.038.3979953
TCAACGC4700.037.9895064
CAACGCA4500.037.589625
GCGATTA250.001702370237.58961580-81
AACGCAG4750.034.6220176
ATGGGTA1107.640301E-834.172385
TATCAAC5600.032.733862
ATCCTAT600.004148684431.3349761
GGTACCT1351.3147655E-831.3246828
TTATACA753.2675973E-431.3246824
ACGCAGA5250.031.324687
CGCAGAG5550.029.6314548
CGATTAC404.7804473E-429.3668980-81
TGGGTAC1303.307614E-728.9150926
TCCGCGG350.00872517126.92047746-47
Produced by FastQC (version 0.11.2)