FastQCFastQC Report
Sun 14 Apr 2019
SRR6576220_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576220_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152205
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3610.23718011891856378No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2470.16228113399691207No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2430.15965309943825762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.11169146874281397No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1670.10972044282382314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA3550.059.6091351
ATCAACG4900.041.239713
TCAACGC4900.041.239714
CAACGCA5000.040.4149135
AACGCAG5450.037.9401786
TACCACG500.001698441637.5952727
GTATACC500.001698441637.5952724
ACGCAGA5800.035.6506847
CGCAGAG5950.035.5651178
TATCAAC6000.034.4623342
CTTCTAA550.002702586634.1999861
GGTATCA1309.029463E-932.555761
GCAGAGT6600.032.062499
CCGGTCC600.00413896631.3499879
CTAACTC600.0041523131.3293913
GTACATG7150.030.9115281
TACATGG7550.029.8770352
ACATGGG7600.029.6804773
AGAGTAC6300.027.24463510-11
TTAGATG700.00881780226.8537643
Produced by FastQC (version 0.11.2)