FastQCFastQC Report
Sun 14 Apr 2019
SRR6576221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180316
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3930.2179507087557399No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2320.12866301382018233No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1990.11036180926817364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.10814348144368775No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1820.10093391601410856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG301.3662051E-462.6836781
AAAACGC250.005229025656.3996663
GGACGTT250.005229025656.3996666
GTCTATG352.9269312E-453.7288671
GTATAAC555.256891E-542.7388731
ATGGGTA1400.040.2854735
ACTTATT704.61369E-640.2854739
GTATCAA5000.039.490721
TGCCCGC500.001695960637.6102071
TATTCTG1251.4915713E-1037.599785
GACTTAT757.409086E-637.5997778
AACGCAG7250.035.0066875
TACATGG11850.033.7222332
ACATGGG11850.033.7128833
GTACATG11900.033.5805441
ATCAACG7450.032.8142742
TTATTCT1309.096766E-932.538274
TCAACGC7800.031.9357093
CATGGGA5500.031.6179964
CAACGCA7600.031.5392884
Produced by FastQC (version 0.11.2)