Basic Statistics
Measure | Value |
---|---|
Filename | SRR6576221_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 180316 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG | 403 | 0.22349652831695466 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 344 | 0.19077619290578762 | No Hit |
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA | 281 | 0.15583752967013464 | No Hit |
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA | 256 | 0.14197298076709777 | No Hit |
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA | 249 | 0.13809090707424745 | No Hit |
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG | 228 | 0.12644468599569644 | No Hit |
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT | 203 | 0.11258013709265954 | No Hit |
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG | 191 | 0.10592515361920185 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTAGTAC | 20 | 0.0021365953 | 70.69397 | 1 |
GTATAAC | 50 | 2.950928E-5 | 47.12931 | 1 |
GTATCAA | 590 | 0.0 | 44.732903 | 1 |
TGTAGCG | 45 | 0.001011827 | 41.776543 | 2 |
GTACATG | 1300 | 0.0 | 39.15358 | 1 |
TACATGG | 1325 | 0.0 | 38.66301 | 2 |
ATGGGAC | 110 | 1.7789716E-9 | 38.453407 | 5 |
ACATGGG | 1325 | 0.0 | 37.953594 | 3 |
CATGGGA | 590 | 0.0 | 37.43957 | 4 |
CAACGCA | 750 | 0.0 | 36.345596 | 5 |
AACGCAG | 740 | 0.0 | 36.201633 | 6 |
ATCAACG | 755 | 0.0 | 34.8599 | 3 |
TCAACGC | 780 | 0.0 | 34.34514 | 4 |
CATGGGT | 275 | 0.0 | 34.18081 | 4 |
GGTACCT | 165 | 8.54925E-11 | 31.332409 | 8 |
CATGGGG | 520 | 0.0 | 30.729862 | 4 |
ATGGGAT | 185 | 9.094947E-12 | 30.485586 | 5 |
GGGTACC | 185 | 9.094947E-12 | 30.485586 | 7 |
TATCAAC | 880 | 0.0 | 29.908207 | 2 |
ACGCAGA | 880 | 0.0 | 29.908207 | 7 |