FastQCFastQC Report
Sun 14 Apr 2019
SRR6576221_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576221_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180316
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4030.22349652831695466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.19077619290578762No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2810.15583752967013464No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2560.14197298076709777No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2490.13809090707424745No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2280.12644468599569644No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT2030.11258013709265954No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1910.10592515361920185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAC200.002136595370.693971
GTATAAC502.950928E-547.129311
GTATCAA5900.044.7329031
TGTAGCG450.00101182741.7765432
GTACATG13000.039.153581
TACATGG13250.038.663012
ATGGGAC1101.7789716E-938.4534075
ACATGGG13250.037.9535943
CATGGGA5900.037.439574
CAACGCA7500.036.3455965
AACGCAG7400.036.2016336
ATCAACG7550.034.85993
TCAACGC7800.034.345144
CATGGGT2750.034.180814
GGTACCT1658.54925E-1131.3324098
CATGGGG5200.030.7298624
ATGGGAT1859.094947E-1230.4855865
GGGTACC1859.094947E-1230.4855867
TATCAAC8800.029.9082072
ACGCAGA8800.029.9082077