FastQCFastQC Report
Sun 14 Apr 2019
SRR6576222_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576222_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161609
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4160.2574114065429524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.20667165813785124No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2390.14788780327828277No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2240.1386061419846667No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1960.12128037423658336No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT1930.11942404197786015No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1790.11076115810381847No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1730.10704849358637206No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1710.10581093874722325No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG1700.10519216132764883No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1690.10457338390807441No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1650.10209827422977681No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1650.10209827422977681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG250.00517970956.532131
GATAGTC250.005230786356.3922047
GTATCAA6350.040.8040351
GGGTACC1601.8189894E-1235.2451257
GTACATG11400.033.886221
TACATGG11350.033.5372162
CATGGGG4750.032.6481174
ACATGGG11650.031.463463
ATGGGGA2750.030.7593845
ATCAACG7950.030.1467723
AACGCAG7700.029.9049596
CAACGCA7900.029.7427235
TCAACGC7900.029.7427234
CCATTAT804.698069E-429.4438191
ATACTGT804.766789E-429.370946
TATCAAC8600.028.4146772
CGCAGAG8350.027.5940998
GGTACCT1555.003494E-827.3034448
ACGCAGA8450.027.2506717
CATTATT700.00881991826.853432
Produced by FastQC (version 0.11.2)