FastQCFastQC Report
Sun 14 Apr 2019
SRR6576223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209723
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6510.31040944483914495No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3510.16736361772433161No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3460.1649795206057514No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3340.15925768752115885No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3150.15019811847055403No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2780.13255579979306037No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2770.13207898036934435No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2630.1254035084373197No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2420.11539030053928276No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2370.11300620342070256No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT2300.10966846745469024No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2280.10871482860725815No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2250.10728437033611No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10585391206496188No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2130.10156253725151748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT250.00523066856.39842294
ATAAGGT405.666827E-446.9986883
GTATCAA6900.040.2064551
AATACAC608.800867E-539.1655735
GTACTAC651.4058324E-436.1700861
GTATAAG651.4058324E-436.1700861
GTACATG14550.035.8717771
ACATGGG14550.035.5316543
TACATGG14950.034.9119952
CATGGGG6600.034.1808664
TATAGGG600.004144177331.3474062
CTTAGAC600.004153867331.3324583
TTACACC600.004153867331.3324584
ATGGGGA3500.030.8848535
AACGCAG8700.030.252036
TTCTAGG650.00612938828.936072
TGCACTC650.006143694328.922275
CAACGCA9350.028.1489475
CATGGGT3700.027.9451664
ATCAACG9300.027.7949243
Produced by FastQC (version 0.11.2)