FastQCFastQC Report
Sun 14 Apr 2019
SRR6576223_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576223_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209723
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5740.2736943492130095No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3150.15019811847055403No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2950.1406617299962331No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2890.13780081345393685No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2740.1306485220981962No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2580.12301941131873947No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2490.11872803650529508No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2480.11825121708157903No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2420.11539030053928276No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2380.1134830228444186No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2370.11300620342070256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10490027321752979No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT2100.10013207898036934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG250.005230719756.3982853
TTGTGTA652.7745755E-643.3832979
GTATAAT1107.456947E-834.278861
GTCTTAG550.002674517234.278861
GTCTTAA753.2126045E-431.4222851
CATGGGT3450.031.332384
GTACATG13750.030.5081831
TACATGG13800.029.9701022
CATGGGG5200.029.8260174
ACATGGG13750.029.7372763
GTATTAG804.6891905E-429.4583931
CTATAAT804.6891905E-429.4583931
AATTGCT650.0061437728.92227
ATGGGTA2002.5465852E-1128.1991425
GGTACCT1853.237801E-1027.9450958
GTATCAA7750.027.3677981
TATGACT700.00882116326.8563274
CTTATGT700.00882116326.8563273
TATCCCT350.00883104926.85632774-75
TAGAGGA909.493348E-426.1103174
Produced by FastQC (version 0.11.2)