FastQCFastQC Report
Sun 14 Apr 2019
SRR6576225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445985
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13400.30045853560097313No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7930.17780867069520276No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7780.17444532887877395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.16345841227843988No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7160.1605435160375349No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7130.15987084767424914No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7030.15762861979662993No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6890.15448950076796306No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6420.1439510297431528No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6300.14126035629000974No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6230.1396907967756763No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5950.13341255871834254No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT5610.12578898393443724No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC5430.1217529737547227No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5310.11906230030157965No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5280.1183896319382939No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5200.11659584963619853No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG5190.1163716268484366No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5080.11390517618305548No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5070.11368095339529356No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5060.11345673060753166No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC4930.11054183436662668No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4770.10695426976243595No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4730.10605737861138828No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4690.1051604874603406No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4670.10471204188481677No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4670.10471204188481677No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC4610.10336670515824524No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4580.10269403679495946No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG4510.10112447728062603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA156.8999105E-493.998324
CGCTAAA200.002162262870.498735
CCGAACT250.00521807456.4432871
TCTATAC250.005231993756.405312
GCCCGCA352.9341917E-453.719352
GTACTTG2300.049.0811231
CCGCAAA405.673461E-446.9991574
ACTTGGG2500.045.119193
GTATCAA14550.042.0253561
GTTCTAG450.001009468641.809841
CCCGCAA450.001013376941.7770273
GACATAT1051.1277734E-940.3166351
TGCCCGC853.924706E-738.735591
TGCATGC500.001701060437.5993237
AGGTATA1152.7685019E-936.781956
GGTACCT4250.035.3876048
ATGGGTA5800.034.8442045
TATACCG550.002715471734.1812064
TACCGGG550.002715471734.1812066
ATCAACG17450.033.3976823
Produced by FastQC (version 0.11.2)